Characterization of the Pho89 phosphate transporter by functional hyperexpression inSaccharomyces cerevisiae
نویسندگان
چکیده
منابع مشابه
Functional expression, purification and reconstitution of the recombinant phosphate transporter Pho89 of Saccharomyces cerevisiae
The Saccharomyces cerevisiae high-affinity phosphate transporter Pho89 is a member of the inorganic phosphate (Pi) transporter (PiT) family, and shares significant homology with the type III Na(+)/Pi symporters, hPit1 and hPit2. Currently, detailed biochemical and biophysical analyses of Pho89 to better understand its transport mechanisms are limited, owing to the lack of purified Pho89 in an a...
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The extracellular phosphate concentration permissive for the expression of different amounts of the active high-affinity Pho84 phosphate transporter in the plasma membrane as well as the PHO84 messenger RNA levels in low-phosphate-grown Saccharomyces cerevisiae cells is very narrow and essential for a tight regulation of the transporter. The Pho84 transporter undergoes a rapid degradation once ...
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ژورنال
عنوان ژورنال: FEMS Yeast Research
سال: 2008
ISSN: 1567-1356,1567-1364
DOI: 10.1111/j.1567-1364.2008.00408.x